Taurine amino acid supplementation impacts performance, blood hematology, oxidative stress, and jejunum morphology in broiler chickens.

Excess levels of free radicals cause oxidative damage to cells. Taurine is a rare amino acid with antioxidant effects whose dietary deficiency increases oxidative damage to the cell membrane. To investigate the effects of dietary taurine supplementation on performance, blood hematology, oxidative stress, and jejunum morphology in broilers, 300 broilers (Ras 308, 1D of age) were randomly allocated into 4 groups with 5 replicates of 15 birds. The experimental treatments included basic diet (control treatment) and basic diet with 1, 3, and 6 g/kg taurine amino acid. During 1 to 45 days, the inclusion of taurine supplementation in diets improved the body weight gain (BWG), feed consumption (FC), and feed conversion ratio (FCR) of broilers (P < 0.05). In CBC tests, the experimental treatments were significantly different concerning the red blood cell (RBC) count, the average hemoglobin in the cell, the RBC width in the curve, and the hematocrit (P < 0.05). Despite the significance of oxidative stress among the treatments, the control and fourth treatments showed the highest and the lowest oxidative stress, respectively (P < 0.05). Also, in jejunum morphology, the fourth treatment showed the best performance in terms of villus length and width and the villus length to crypt depth (V/C) ratio (P < 0.05). Overall, 6 g/kg taurine addition to the diet reduced oxidative stress and positive features in the jejunum morphology while improving the functional traits of broilers.

Feeding strategy and prebiotic supplementation: Effects on immune responses and gut health in the early life stage of broiler chickens.

This study aimed to investigate the effects of early or late feeding strategies and prebiotic, on immune responses and gut health during the early life stage of broiler chickens. A total of 240 day-old male broiler chicks were used in a 2 × 3 factorial arrangement of treatments that comprised 2 feeding strategies (early or late) and 3 levels of prebiotic (0, recommended dosage or three times the recommended dosage) in a completely randomized design with 4 pen replicates and 10 broilers per each. Compared to broiler chickens that had early access to feed, delayed access to feed resulted in an increased population of Escherichia coli and a decreased population of Lactobacillus spp. and Bifidobacterium spp. in the ileum (P < 0.05). Additionally, delayed access to feed led to a decrease in villus height, crypt depth, villus height: villus width ratio, goblet cell density, and mucin 2 gene expression in the ileum (P < 0.05). The supplementation of prebiotics in both the late and early feeding strategy groups resulted in increased villus height, crypt depth, goblet cell density, mucin 2 gene expression, and antibodies against Infectious Bursal Disease (IBD). Additionally, it led to an improvement in the foot web thickness index (P < 0.05). Furthermore, it resulted in a significant decrease in the population of Escherichia coli, while the populations of Lactobacillus spp. and Bifidobacterium spp. in the ileum were significantly increased (P < 0.05). Therefore, this study suggests that incorporating prebiotics in the starter diet can effectively enhance immune responses and promote gut health, regardless of the feeding strategy (early or late). In conclusion, this study demonstrates the potential benefits of incorporating prebiotics into poultry diets to alleviate the detrimental effects of delayed access to feed and improve gut health during the early life stage of broiler chickens.

Effect of exogenous genistein on osteogenic differentiation of adipose-derived mesenchymal stem cells in laying hens.

Previous literature revealed that genistein might play a preventive role in osteoporosis. Therefore, we aimed to evaluate the effect of genistein on the osteogenic potency of laying hens' adipose-derived stem cells (LHASCs). The viability of LHASCs after isolation was investigated on tissue culture plastic (TCP) under exposure to genistein up to 50 µg/mL by MTT assay. Our preliminary result revealed that LHASCs cultured under genistein exposure up to 20 µg/mL are feasible. Then, we evaluated the osteogenic induction of LHASCs under exposure to 0, 10, and 20 µg/mL genistein. The Alizarin Red staining confirmed the calcium deposition. Our findings showed that osteogenic differentiation under exposure to 20 µg/mL genistein led to higher ALP activity and more calcium content. We then tried to see the probable additive effect of the genistein-plus Poly-L-lactic acid (PLLA) scaffold on the cell viability and osteogenic capacity of LHASCs. For this, cells were cultured on a PLLA scaffold and exposed to 20 µg/mL genistein. Cell growth rate, as indicated by the MTT assay, revealed no differences between the groups. LHASCs cultured on a genistein-plus PLLA scaffold showed higher ALP activity and more calcium content. The expressions of Osteocalcin, COL1A2, ALP, and Runx2 genes were increased in the genistein-plus PLLA group as compared with PLLA and TCP groups. Adequate proliferation rates and higher expression of osteogenic markers provide genistein as a suitable substrate to support the proliferation and differentiation of LHASCs. Genistein supports osteogenic induction as a further positive effect if such a PLLA scaffold is available.

Effects of sesame meal bioactive peptides, individually or in combination with a mixture of essential oils, on growth performance, carcass, jejunal morphology, and microbial composition of broiler chickens.

The objective of the current study was to investigate the influence of sesame meal bioactive peptides (SMBP) individually or in combination with a mixture of savory (Satureja khuzestanica) and thyme (Thymus daenensis) essential oils (STEO) on growth performance, carcass, jejunal morphology, and cecal microbial composition of broiler chickens. A total number of 250-day-old male Ross broiler chicks were randomly allocated to 5 dietary treatments with 5 replicates per each. Dietary treatments were a corn-soybean meal diet as control; control diet supplemented with 0.5 g/kg of Bacitracin as antibiotic (A); 3 g/kg of SMBP; 0.5 g/kg of STEO; and a combination of 3 g/kg of SMBP and 0.5 g/kg of STEO. Results indicated that inclusion of SMBP + STEO in diet improved body weight gain and feed conversion ratio during 11 to 24 days (P < 0.05). Dietary treatments had no significant effect on the carcass characteristics and internal organs. In jejunal morphology, the villus length (VL) and the ratio of VL to crypt depth (CD) were greater in birds which received SMBP + STEO diets, while CD was lower in broilers fed with SMBP + STEO diet (P < 0.05). Inclusion of SMBP in combination with STEO increased viable count of Lactobacillus while the population of E. coli decreased in birds fed with SMBP + STEO diet (P < 0.05). According to the results of this experiment, it can be concluded that dietary SMBP in combination with STEO had positive effects on the growth performance, jejunal morphometric indices, and cecal microbial composition of broiler chickens.

Growth performance, internal organ traits, intestinal morphology, and microbial population of broiler chickens fed quinoa seed-based diets with phytase or protease supplements and their combination.

The aim of this study was to evaluate the effects of quinoa seed-based diets with phytase and protease enzymes, individually or in combination, on the productive performance, internal organs, microbiota activity, and intestinal morphology of broiler chickens. A total of 250 1-day-old broiler chicks (ROSS 308) were randomly distributed into five treatments with each five replicate pens of 10 birds in a 42-day trial. Dietary treatments were a conventional diet (CC) based on a corn-soybean meal or an experimental diet (EC) which contained 150 g/kg of quinoa seed. Other treatments were EC diet supplemented with 500 FTU/kg phytase (EPH), 0.2 g/kg protease (EPR) enzymes, or their combination (EPPC). At the end of the trial, the feed intake of the EPH broilers was higher than those of the EC and EPPC birds (P < 0.05). Meanwhile, broiler chickens fed the EPH and EPPC diets had higher body weight gain compared with those of the birds fed the other diets (P < 0.05). The EC and EPPC broilers consumed less feed than the EPH birds (P < 0.05), while CC and EPR birds were intermediate. The EC group had the highest feed conversion ratio among all groups (P < 0.05). The EPPC group had the best feed conversion ratio compared with other groups except for the EPH group (P < 0.05). The highest relative weight of the bursa and spleen was observed in EPPC and CC groups, respectively. The pancreas had the lowest weight in the broilers fed protease-supplemented diet. An increase in villus length and villus width was observed in birds fed EPH and EC diets, respectively (P < 0.05). The ratio of VL to crypt depth was greater in the phytase-supplemented group (P < 0.05). Inclusion of phytase or phytase + protease to QS diet increased the cecal Lactobacillus population, while the count of Coliforms decreased in EPR and EPPC groups (P < 0.05). It is concluded that addition of phytase and protease enzymes to QS-based diet may have beneficial effects on the growth performance, gut bacteria ecology, and intestinal morphology of broiler chickens.

Efficacy of phytobiotic and toxin binder feed additives individually or in combination on the growth performance, blood biochemical parameters, intestinal morphology, and microbial population in broiler chickens exposed to aflatoxin B1.

This experiment was conducted to investigate the effects of phytobiotic and antifungal feed additives on the growth performance, blood parameters, intestinal morphology, and cecal microbiota activity of broiler chickens under aflatoxicosis challenge. A total of 250 one-day-old Ross 308 broiler chicks (mixed sex) were reared on the littered floor with a completely randomized design by five treatments and five replicates for 35 days. Treatments included positive control (without AFB1), negative control-AFB1 (1 ppm), negative control-phytobiotic (Entex, 0.5 kg/t), negative control-Mycofix Plus (0.5 kg/t), and negative control-phytobiotic + Mycofix Plus. Dietary phytogenic and toxin binder improved body weight gain and feed conversion ratio of broiler chickens (p<0.05). Serum concentration of AST increased in broilers which received AFB1 without additives, while the blood concentration of total protein decreased (p<0.05). In jejuna morphometric indices, it was observed that the broiler chickens fed phytobiotic additive in combination with toxin binder had a greater villus length and crypt depth (p<0.05). Dietary treatments had no significant effect on the cecal microbial population in broiler chickens. In conclusion, the present results indicated that phytobiotic and toxin binder supplement improved growth performance and intestinal morphology of broiler chickens exposed to AFB1 challenge.